Two-photon MINFLUX with doubled localization precision
نویسندگان
چکیده
Abstract Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to nanoscopy. MINFLUX pioneers this transition through point spread function (PSF) engineering, yet its performance is primarily limited by the signal-to-background ratio. Here we demonstrate theoretically that two-photon (2p-MINFLUX) could double PSF engineering nonlinear effect. Cramér-Rao Bound (CRB) studied as maximum precision, and CRB halved compared single-photon (1p-MINFLUX) in all three dimensions. Meanwhile, order achieve same 1p-MINFLUX, 2p-MINFLUX requires only 1/4 photons. Exploiting simultaneous excitation multiple fluorophore species, may have potential registration-free nanoscopy multicolor tracking.
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ژورنال
عنوان ژورنال: eLight
سال: 2022
ISSN: ['2662-8643']
DOI: https://doi.org/10.1186/s43593-021-00011-x